SpikeGLX Tutorial
Graphs Window: Tune for Spikes
Graphs Window: Tune for Spikes
•Explore freely –saved data not affected
•Default settings can’t see anything
•Set Yscl and band pass
•Set BinMax and Gbl Dmx
•Set shorter time span
•Open ShankViewer
•Open SpikeViewer
Data: 2.0 probe in rat hippocampus.
Default settings below.
Can’t see anything!!
Double-click on channel zero.
Hover cursor near top of graph and read off voltage range = +/-6.2 mV.
We need to magnify to see spikes we expect are ~200 uV.
Change Yscl to 10.
If traces have DC offsets, click -<T> to remove that.
NP 2.0 is full band, switch ‘AP Native’ to ‘300-INF’.
Now we can see some tiny negative ticks…better.
Click BinMax to show hidden peaks:
4 seconds of data is 120K points but monitor ~1000 pixels wide
data downsampled to fit. BinMax makes sure we keep data
points with larger voltages so we don’t miss spikes.
But this also enhances noise.
So turn on -<S> = Gbl Dmx, a CAR filter.
Set Sec to 0.1 seconds to ‘zoom in’ on spike events.
Double-click a channel, and if it has spikes, they look like this.
But how to find hot channels?
Click open the ShankViewer.
Initially, can’t see much
shank length
-> set 3 pix/pad.
AP pk-pk volts was saturated
with all pads white
-> set 400 uV scale.
Now differentiated.
Active channels are bright.
Click on bright area of shank
to select that channel in
graphs.
Click on graph to select that
channel on shank, they are
linked.
Right-click on either a graph trace, or a pad in the ShankViewer.
In menu, send to SpikeViewer.
You can watch up to 4 channels.
